Journal: Scientific Reports
Article Title: Next-generation Candida albicans vaccine VXV-01 containing recombinant Als3p and Hyr1p antigens for invasive Candida infections
doi: 10.1038/s41598-025-32488-8
Figure Lengend Snippet: The manufacturing processes of C. albicans Als3p and Hyr1p antigens. The flowchart of the Upstream (Upper) and Downstream (Right) manufacturing process of recombinant Als3p ( A) and Hyr1p (B) protein expressed and purified from S. cerevisiae and E.coli , respectively, is outlined in the flow diagram. SDS-PAGE analysis of recombinant Als3p and Hyr1p antigens (C) . Single clean protein bands of each protein indicate high purity and integrity of the antigens. Bovin serum albumin (BSA) was included as a control.
Article Snippet: For Hyr1p expression, an N-terminal region of the Hyr1p gene was bacterial codon-optimized, cloned in a bacterial proprietary plasmid expression vector (Nature Technologies Inc.), and transformed into a competent E. coli BL21 strain (New England Biolabs).
Techniques: Recombinant, Purification, SDS Page, Control